Protective effects of Fu-Yuan-Xing-Nao decoction for focal cerebral ischemia reperfusion injury in rats / 药学实践杂志

ABSTRACT

Objective To study the protective effect of Fu-Yuan-Xing-Nao decoction (FYXND) on rats with middle cere-bral ischemia reperfusion injury .Methods Male Sprague-Dawley rats were randomly divided into sham ,ischemia-reperfusion , and low ,middle ,high FYXND dose (5 .5 ,11 ,22 g/kg) groups .Rats model was induced by 2 h of middle cerebral artery oc-clusion and 24 h reperfusion .The neurological deficit score of each group was evaluated .The infarct size was measured by the 2 ,3 ,5-triphenyltetra-zolium(TTC) chloride staining assay .The pathological changes of brain tissue were observed by HE stai-ning assay .The changes of Nissl bodies were observed by Nissl staining .Tunnel staining was used to observe the apoptosis of neurons in brain .Serum levels of superoxide dismutase (SOD)and Malondialdehyde (MDA ) were measured .Results Com-pared with the model group ,neurological outcomes were improved in all three groups of low ,middle and high FYXND dose (5 .5 ,11 ,22 g/kg) .Significantly reduced infarct brain volume was observed with TTC staining in all three FYXND groups . The results from HE staining assay indicated that the pathological structure of brain tissue was improved in the treatment groups .The numbers and morphology of Nissl corpuscles in the treated group were also improved based on the results of Nissl staining .Both the Tunnel staining positive cells and the rate of apoptosis were decreased .Compared with the model group , FYXND increased the rat serum SOD level and decreased the MDA level .Conclusion FYXND has protective effects on cere-bral ischemia reperfusion injury in rats .