Tumor Establishment Features of Orthotopic Murine Bladder Cancer Models
Korean Journal of Urology
;
: 396-400, 2012.
Artigo
em Inglês
| WPRIM
| ID: wpr-79099
ABSTRACT
PURPOSE:
Animal tumor models are important for the evaluation of novel therapeutic modalities. Since the initial report of an orthotopic bladder tumor model, several modifications have been proposed to improve the tumor take rate. Here we compared the HCl-pretreated and electrocauterization-pretreated orthotopic murine bladder tumor models. MATERIALS ANDMETHODS:
MBT-2 murine bladder cancer cells were transurethrally implanted in the bladder of syngeneic C3H/He mice. The mice were divided into three groups according to pretreatment methods (electrocautery, HCl, and control group) and were subjected to pretreatment before instillation of MBT-2 tumor cells into the bladder. Mice were sacrificed on day 21, and bladders were harvested, weighed, and examined histopathologically.RESULTS:
The tumor take rate of the control, electrocautery, and HCl groups was 0%, 54%, and 100%, respectively. The tumor take rate of the HCl group was significantly higher than that of the control group (p<0.01) and the electrocautery group (p=0.01). Pathologic reports revealed that all established bladder tumors were high-grade papillary urothelial carcinomas.CONCLUSIONS:
The HCl pretreatment model was a preferable murine bladder tumor model for evaluating further therapeutic interventions.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bexiga Urinária
/
Neoplasias da Bexiga Urinária
/
Administração Intravesical
/
Modelos Animais
/
Eletrocoagulação
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Journal of Urology
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS