Effects of miR-361-5p on malignant biological behaviors of renal cell carcinoma ACHN cells / 中国肿瘤生物治疗杂志

ABSTRACT

@#Objective: To investigate the effect of miR-361-5p on proliferation, invasion, migration, apoptosis and cell cycle of renal cell carcinoma ACHN cells. Methods: MiR-361-5p mimics and miR-361-5p inhibitor were transfected into ACHN cells, respectively. The expression of miR-361-5p in the transfected cells was detected by Real-time quantitatibse PCR; and the proliferation, migration, invasion, cell cycle and apoptosis of cells were detected by MTT assay, Scratch-healing assay, Transwell assay and flow cytometry, respectively. Results: Comparedwiththecontrolgroupandmimics-NCgroup,theexpressionofmiR-361-5pwas increased significantly in ACHN cells of -miR-361-5p mimics group (P<0.01), the abilities of cell proliferation, invasion and migration were decreased significantly (all P<0.01), and the apoptosis rate was increased (P<0.01). Compared with the control group or inhibitor-NC group, the expression of miR-361-5p was significantly decreased in ACHN cells of miR-361-5p inhibitor group (P<0.01), the abilities of cell proliferation, invasion and migration were increased (all P<0.01), the cell cycle was accelerated (P<0.01), and the apoptosis rate was decreased (P<0.05). Conclusion: miR-361-5p can inhibit the proliferation, invasion and migration of renal cell carcinoma ACHN cells, and induce cell apoptosis, which plays an important inhibitory role in the development of renal cell carcinoma.