Association of Killer Cell Ig-like Receptor (KIR) with an Adaptor Protein Shc
Immune Network
;
: 67-75, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-79622
ABSTRACT
BACKGROUND:
Cytotoxic function of killer cells is inhibited by specific recognition of class I MHC molecules on target cells by inhibitory killer Ig-like receptors (KIR) expressed on NK cells and some cytotoxic T cells. The inhibitory effect of KIR is accomplished by recruitment of SH2-containing protein tyrosine phosphatase (SHP) to the phosphotyrosine residues in the cytoplasmic tail.METHODS:
By in vitro coprecipitation experiments and transfection analysis, we investigated the association of KIR with an adaptor protein Shc in Jurkat T cells.RESULTS:
The cytoplasmic tail of KIR appeared to associate with an adaptor protein Shc in Jurkat T cell lysates. Similar in vitro experiments showed that phosphorylated KIR cytoplasmic tail bound SHP-1 and Shc in Jurkat T cell lysates. The association of KIR with Shc was further confirmed by transfection analysis in 293T cells. Interestingly, however, Shc appeared to be replaced by SHP-2 upon engagement of KIR in 293T cells.CONCLUSION:
Our data indicate that KIR associate with an adaptor protein Shc in Jurkat T cells, and suggest that KIR might have an additional role which is mediated by this adaptor protein.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células Matadoras Naturais
/
Linfócitos T
/
Transfecção
/
Proteínas Tirosina Fosfatases
/
Fosfotirosina
/
Citoplasma
/
Proliferação de Células
Idioma:
Inglês
Revista:
Immune Network
Ano de publicação:
2006
Tipo de documento:
Artigo
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