Construction of Recombinant DNA with F and HN Genes of Newcastle Disease Virus and Its Immunogenicity
Journal of Bacteriology and Virology
;
: 99-107, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-79627
ABSTRACT
Recombinant DNA vaccines, based on plasmid vectors expressing an antigen under the control of a strong promotor, have several advantages over traditional vaccines. They have been shown to induce a full spectrum of immune responses for humoral and cellular systems and to secure the higher safety and the simplicity of administration. Thus, establishment of DNA vaccines against Newcastle disease virus (NDV) in poultry has been widely investigated using various virus strains and vector systems. In this study, the F and HN genes of NDV CBP-1 strains isolated from diseased pheasants and attenuated by serial passages in egg embryos were cloned using pSLIA vector and constructed two recombinants of pSLIA-tsF and pSLIA-tsHN. The recombinant plasmids were transfected into COS-7 cell and the expression of HN and F proteins were verified by immunofluorescence, SDS-PAGE and Western blot. The recombinant plasmids were injected intramuscularly and intradermally into C57B/6 mouse and a significant increment of HN and F antibodies was detected by ELISA. According to the results, it was implicative that the recombinant DNA could be utilized for development of recombinant DNA vaccine for NDV.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Óvulo
/
Plasmídeos
/
Aves Domésticas
/
Vírus da Doença de Newcastle
/
DNA Recombinante
/
Ensaio de Imunoadsorção Enzimática
/
Inoculações Seriadas
/
Vacinas
/
Western Blotting
/
Imunofluorescência
Limite:
Animais
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS