Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
The Korean Journal of Parasitology
; : 353-355, 2013.
Article
em En
| WPRIM
| ID: wpr-79736
Biblioteca responsável:
WPRO
ABSTRACT
There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Polimorfismo de Fragmento de Restrição
/
Reação em Cadeia da Polimerase
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Sensibilidade e Especificidade
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Criptosporidiose
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Cryptosporidium
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Genótipo
Tipo de estudo:
Diagnostic_studies
Limite:
Child
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Female
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Humans
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2013
Tipo de documento:
Article