High intensity focused ultrasound inhibits the proliferation and migration of pancreatic cancer cells via regulating miR-1297/PTEN axis / 中国肿瘤生物治疗杂志

ABSTRACT

@# Objective: To explore the role and molecular mechanism of high intensity focused ultrasound (HIFU) inhibits pancreatic cancer cell proliferation and invasion via regulating miR-1297/PTEN axis. Methods: With treating the cells with HIFU for 1 to 3 seconds, the effect of HIFU on cell proliferation, invasion and apoptosis of PANC-1 cells in vitro was examined by CCK-8, Transwell and Annexin V-FITC/PI double staining flow cytometry, respectively. The effect of HIFU on expression of miR-1297 and PTEN were measured by qPCR and Western blotting. Moreover, the relationship between miR-1297 and PTEN was examined by dual luciferase report assay. Western blotting was used to detect the effect of HIFU on the expression of Akt, p-Akt (308) and p-Akt (473) after transfected with miR-1297 inhibitor and PTEN-siRNA. Results: HIFU treatment significantly inhibited the cell proliferation, invasion and promoted apoptosis of PANC-1 cells (all P<0.01), which was associated with inhibition of miR-1297 expression and activation of PTEN expression in pancreatic cancer cells (all P<0.01). Moreover, miR-1297 directly binds to the 3′ UTR of PTEN mRNA to suppress its expression in PANC-1 cells. Further, HIFU exposure could significantly inhibit the expression of the phosphorylation of Akt (P<0.01). Conclusion: HIFU inhibits PTEN and blocksAkt signaling by down-regulating miR-1297, thereby suppresses the occurrence and progress of pancreatic cancer.