Study on Immunoregulatory Effect of Compatibility of Salviae Miltiorrhizae Radix et Rhizoma Combined with Sophorae Flavescentis Radix in Immunosuppressive Mice / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
;
(24): 60-65, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-802269
ABSTRACT
Objective:
To investigate the mechanism of immunomodulatory effect of extracts from Salviae Miltiorrhizae Radix et Rhizoma and Sophorae Flavescentis Radix on immunodeficiency mice by immunosuppressive mouse model induced by cyclophosphamide.Method:
An immunosuppressive animal model was established by cyclophosphamide. The blank group, the model group, the low,medium and high dose group of Salvia miltiorrhiza and Sophora extract groups (25,50,100 mg·kg-1), using mouse organs organ evaluation index using a mouse model to evaluate the carbon clearance phagocytic cell function using luminex to detect levels of relevant cytokines in serum and using flow to detect the number of helper T cells. Mouse mononuclear macrophage leukemia cells (RAW264.7) were cultured in vitro, and the effect of Salvia miltiorrhiza extracts on the proliferation of RAW264.7 cells were detected by methylthiazolyldiphenyl-tetrazolium bromide(MTT)assay. The proliferation was induced by Real-time PCR. The impact and its possible mechanisms are explored.Result:
Compared with blank group, cyclophosphamide significantly inhibited liver index (PPPP-1 cyclophosphamide significantly inhibited the proliferation of mononuclear macrophage RAW264.7 (PPPPPPPP1 gene (CCND1) plays an anti-apoptotic role.Conclusion:
The extracts from Salviae Miltiorrhizae Radix et Rhizoma and Sophorae Flavescentis Radix can counteract the immunosuppression caused by cyclophosphamide and enhance the immune function of animals.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Experimental Traditional Medical Formulae
Ano de publicação:
2019
Tipo de documento:
Artigo
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