Analysis of epidemic characteristics of rotavirus diarrhea in children under 5 years in Lanzhou, 2017 / 中华实验和临床病毒学杂志

ABSTRACT

Objective@#To analyze the infection status and pathogenic characteristics of rotavirus in group A of children under 5 years of age in Lanzhou city in 2017, and provide scientific data for prevention and control of rotavirus infection.@*Methods@#A total of 219 stool samples from children with diarrhea under the age of 5 years in the Department of Pediatrics, First Hospital of Lanzhou University from January to December in 2017 were collected. A group of human rotavirus positive samples were detected by ELISA, and G/P typing was detected by using reverse transcription-polymerase chain reaction (RT-PCR)) combined with sequencing. Some of the VP7 gene fragments were amplified, sequenced and analyzed for phylogenetic characteristics.@*Results@#A total of 113 rotavirus in group A positive samples were detected, with a total positive rate of 51.60%. The main target of infection was children 0 to 17 months, and the peak of the epidemic was mainly in November. G/P genotyping was performed G9 was predominant G genotype (90.27%), followed by G2 (7.08%). P[8] was predominant P genotype (91.15%), followed by P [4] (7.96%). In 2017, the group A human rotavirus epidemic strain was mainly G9P[8](88.50%). Sequence analysis and phylogenetic analysis of VP7 gene fragments showed that the dominant genotypes G9 had higher homology with isotype reference strains in other regions of China.@*Conclusions@#Children 0 to 17 months old in Lanzhou city are at high risk of group A human rotavirus infection. G9P[8] is a dominant genotype.