Determination of diphenylmethane diisocyanate in workplace air by HPLC with impregnated filter membrane / 中华劳动卫生职业病杂志

ABSTRACT

Objective@#To develop a method for determining diphenylmethane diisocyanate in workplace air by HPLC with impregnated filter membrane.@*Methods@#MDI in workplace air reacted with 1- (2-pyridyl) piperazine on impregnated filter membrane to form MDI-urea derivatives, after elution and filtration, it was detected by HPLC-UV.@*Results@#Limit of detection was 0.003 8 μg/ml and limit of quantification was 0.013 μg/ml. Good linearity was obtained in the range of 0.013~2.000 μg/ml (r=0.999 7) . The precision was 3.10%~8.03% (n=6) , while the recovery was 96.3%~101.9%. Asorption capacity of the membrane was 40.8 μg MDI, and could be stored for 14 days in the light-proof environment of 2~8 ℃.@*Conclusion@#The method optimized testing steps for MDI's standard curve, and provided good guidance for determination of MDI in workplace air with impregnated fiter membrane.