Preliminary study on metabolic symbiosis of HBV-related liver cancer / 中华实验和临床病毒学杂志

ABSTRACT

Objective@#To explore the glycolysis and metabolic symbiosis of HBV-related liver cancer, and to lay the foundation for studying the metabolism of liver cancer cells.@*Methods@#10 cases of HBV-related liver cancer patients were collected, IHC detection of metastatic symbiotic markers MCT1, MCT4 for liver cancer and adjacent tissues; choosing HLE liver cancer cell lines, time gradient group use serum-free DMEM medium, divided into 6 h, 12 h, 24 h and 48 h hypoxia cultivating, by western blotting to detect HIF-1α; oxygen concentration gradient group use serum-free DMEM medium, separate culture in three gas incubator with 0.2 % O2, 8% O2 and 19% O2, by western blotting to detect HIF-1α, GLUT1, PKM2, MCT1 and MCT4.@*Results@#The expression of MCT1 in HBV-related liver cancer tissues was significantly higher than that in adjacent tissues; HLE cell have the highest expression of HIF-1α in the hypoxia cultivating of 12 h. HLE cell have the highest expression of HIF-1α and GLUT1 in hypoxia cultivating of 0.2% O2 and the highest expression of PKM2 in hypoxia cultivating of 8% O2. The oxygen concentration was at 19% MCT1/4 expressed highest.@*Conclusions@#Abnormal energy metabolism in HBV-related liver cancer tissues; the HLE cell is not preferentially choose the metabolism of aerobic glycolysis in an oxygen-free environment or in the near-constant oxygen environment. In hypoxia environment, aerobic glycolysis may also be associated with metabolic symbiosis.