Application and trend of development of digital PCR in virus detection / 中华实验和临床病毒学杂志

ABSTRACT

In recent years, digital PCR (dPCR), as a novel nucleic acid amplification detection technique, contrasts real-time fluorescent quantitative PCR (qPCR) without the need to establish a standard curve, "single molecule template amplification" absolute quantification, insensitive to inhibitors that affect PCR efficiency, and excellent sensitivity, specificity, extremely strong repeatability and the development of the existing commercial platform technology that has great application advantages in virology and nucleic acid quantification. In this review, we address the application advantages of digital PCR in virus detection and describe its future development.