Prognostic Significance of Methylation Profiles in Urothelial Carcinomas of the Bladder
Korean Journal of Pathology
;
: 623-630, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-80796
ABSTRACT
BACKGROUND:
Study on epigenetics of urothelial carcinomas has expanded and allowed better understanding of their correlation with clinicopathologic features. The aim of this study was to determine reliable predictive epigenetic markers for patients with urothelial carcinoma of urinary bladder.METHODS:
In 64 urothelial carcinomas of the urinary bladder, methylationspecific polymerase chain reaction with RAS association domain family 1A (RASSF1A), adenomatous polyposis coli (APC), death-associated protein-kinase (DAPK), runt-related transcription factor 3 (RUNX3), p14, p16 and MGMT was performed and correlated the results with p53 mutations, DNA ploidy, clinicopathologic parameters and recurrences.RESULTS:
Hypermethyation of RASSF1A, APC, DAPK, RUNX3, p14, p16 and MGMT promoters was observed in 35 (54.7%), 29 (45.3%), 18 (28.1%), 18 (28.1%), 9 (14.1%), 2 (3.1%), and 6 (9.4%) cases, respectively. Hypermethylation of RUNX3 and APC was significantly associated with high histologic grades and aneuploidy. Methylation of DAPK was significantly associated with muscle invasion. Methylation of DAPK and RUNX3 genes was significantly associated with recurrence. In survival analyses, methylation of RUNX3 gene and methylation-high (methylation at two or more loci) phenotype was significantly associated with poor recurrence-free survival.CONCLUSIONS:
Methylation of RUNX3 gene and methylation-high phenotype are significant indicator of recurrence.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Ploidias
/
Prognóstico
/
Recidiva
/
Bexiga Urinária
/
DNA
/
Reação em Cadeia da Polimerase
/
Genes Supressores de Tumor
/
Polipose Adenomatosa do Colo
/
Fator 3 de Transcrição
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Korean Journal of Pathology
Ano de publicação:
2010
Tipo de documento:
Artigo
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