Molecular diagnosis of yellow fever imported into Fujian province, 2016 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 441-445, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-808656
ABSTRACT
Objective@#To make laboratorial diagnosis of imported yellow fever (YF) cases in Fujian province with molecular method .@*Methods@#Serum and urine samples were collected from suspected cases at various time-points post illness onset. Real-time RT-PCR and nested RT-PCR were performed respectively for viral specific nucleotide detection and fragment amplification. Sequencing and restrictive fragment length polymorphism (RFLP) method were used to identify the wild virus infection.@*Results@#A total of five cases with wild yellow fever virus (YFV) infection were confirmed in this study. It revealed that the viral agent belonged to Angola-71 like YFV, and the duration of viral agent in urine was longer than that in serum.@*Conclusions@#Simultaneous detection of serum and urine samples would increase detection sensitivity, and further RFLP method contributed to rapid identification of wild YFV infection and exclusion of positive result due to recent vaccination.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2017
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS