Cranial bone regeneration according to different particle sizes and densities of demineralized dentin matrix in the rabbit model / 대한악안면성형재건외과학회지
Maxillofacial Plastic and Reconstructive Surgery
;
: 27-2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-81066
ABSTRACT
BACKGROUND:
The objective of this study was to place bone graft materials in cranial defects in a rabbit model and compare their bone regenerating ability according to the size and density of demineralized dentin matrix (DDM).METHODS:
We selected nine healthy male rabbits that were raised under the same conditions and that weighed about 3 kg. Two circular defects 8 mm in diameter were created in each side of the cranium. The defects were grafted with DDM using four different particle sizes and densities 0.1 mL of 0.25- to 1.0-mm particles (group 1); 0.2 mL of 0.25- to 1.0-mm particles (group 2); 0.1 mL of 1.0- to 2.0-mm particles (group 3); and 0.2 mL of 1.0- to 2.0-mm particles (group 4). After 2, 4, and 8 weeks, the rabbits were sacrificed, and bone samples were evaluated by means of histologic, histomorphometric, and quantitative RT-PCR analysis.RESULTS:
In group 1, osteoblast activity and bone formation were greater than in the other three groups on histological examination. In groups 2, 3, and 4, dense connective tissue was seen around original bone even after 8 weeks. Histomorphometric analysis of representative sections in group 1 showed a higher rate of new bone formation, but the difference from the other groups was not statistically significant. RT-PCR analysis indicated a correlation between bone formation and protein (osteonectin and osteopontin) expression.CONCLUSIONS:
DDM with a space between particles of 200 μm was effective in bone formation, suggesting that materials with a small particle size could reasonably be used for bone grafting.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Osteogênese
/
Tamanho da Partícula
/
Crânio
/
Regeneração Óssea
/
Transplante Ósseo
/
Tecido Conjuntivo
/
Transplantes
/
Dentina
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Maxillofacial Plastic and Reconstructive Surgery
Ano de publicação:
2016
Tipo de documento:
Artigo
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