GTS40, an active fraction of Gou Teng-San (GTS), protects PC12 from HO-induced cell injury through antioxidative properties / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 495-504, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-812088
ABSTRACT
Oxidative stress, a predominant cause of apoptosis cascades triggered in neurodegenerative disorders, has been regarded as a critical inducement in the pathogenesis of Alzheimer's disease (AD). Gou Teng-San (GTS) is a traditional Chinese herbs preparation commonly utilized to alleviate cognitive dysfunction and psychological symptoms of patients with dementia. The present study aimed to investigate the protective effects of GTS40, an active fraction of GTS, on HO-induced oxidative damage and identify the potential active ingredients. Our results revealed that GTS40 exhibited radical scavenging activity, elevated cell viability, decreased the levels of intracellular reactive oxygen species (ROS), and stabilized mitochondrial transmembrane potential (MMP) in HO-treated PC12 cells. In addition, GTS40 blocked the apoptotic cascade by reversing the imbalance of Bcl-2/Bax and inhibiting the activity of caspase-3. Furthermore, an HPLC-QTOFMS method was developed to characterize major chemical constituents in GTS40. Our results revealed twenty-seven identified or tentatively characterized compounds through comparing their retention time (t) and MS spectra with reference standards. These results suggested that GTS40 was a promising active fraction that may be beneficial in the prevention and treatment of oxidative stress-mediated neurodegenerative disorders.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Células PC12
/
Espécies Reativas de Oxigênio
/
Apoptose
/
Estresse Oxidativo
/
Fármacos Neuroprotetores
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Biologia Celular
/
Toxicidade
Tipo de estudo:
Guia de Prática Clínica
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2017
Tipo de documento:
Artigo
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