F-01A, an antibiotic, inhibits lung cancer cells proliferation / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 284-289, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-812264
ABSTRACT
AIM@#In an effort to identify novel, small molecules which can affect the proliferation of lung cancer cells, F-01A, a polyether antibiotic isolated from the fermentation broth of Streptomyces was tested.@*METHOD@#F-01A was tested for its antitumor properties on the lung cancer cell line SPC-A-1, at six doses (0.1, 0.5, 1, 2.5, and 5 μmol·L(-1)), using various cellular assays. Cell viability was measured by the MTT assay, Hochest 33258 was used to study nuclear morphology; DNA ladder and the loss of mitochondrial membrane potential were also evaluated.@*RESULTS@#F-01A induces apoptosis against SPC-A-1 cells in a dose-dependent manner. The IC50 is 0.65 μmol·L(-1), and the inhibition at 5 μmol·L(-1) is 87.89%. Further, JC-1 staining indicates F-01A could induce the loss of mitochondrial membrane potential, and the DNA fragment is evident.@*CONCLUSION@#Mechanistic analysis showed that F-01A induced apoptosis of cancer cells probably in the mitochondrial pathway. The antitumor actions of F-01A involve activation of the apoptotic pathway against SPC-A-1 cells, and it may be valuable for further drug development.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Streptomyces
/
Sobrevivência Celular
/
Química
/
Apoptose
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Potencial da Membrana Mitocondrial
/
Inibidores do Crescimento
/
Neoplasias Pulmonares
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2014
Tipo de documento:
Artigo
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