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In vitro antiproliferative and apoptosis-inducing activities of crude ethyle alcohole extract of Quercus brantii L. acorn and subsequent fractions / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.) ; (6): 196-202, 2016.
Artigo em Inglês | WPRIM | ID: wpr-812436
ABSTRACT
Cancer cell resistance to widely used chemotherapeutic agents is gradually developed. Natural products, mainly isolated from medicinal plants, have been considered as valuable sources for herbal anticancer drugs. The present study aimed to evaluate in vitro antiproliferative and apoptosis-inducing activities of crude ethyle alcohole extract and four fractions of Q. brantii acorn. Crude ethyle alcohole extract of Q. brantii acorn was prepared and subjected to fractionation with different polarity. Subsequently, the extract and the fractions wereevaluated for their in vitro antiproliferative activity in two cancerous (Hela and AGS) and one normal (HDFs) cell lines using MTT [3-(4, 5-dimethylthiazol-2ol) 2, 5 diphenyltetrazoliumbromide] assay. To determine whether the cytotoxicity of these compounds involved the induction of apoptosis, Hela cells were treated with IC50 concentrations of test compounds, stained with both propidium iodide (PI) and Annexin V-fluorescein isothiocyanate (FITC), and analyzed by flow cytometry. In vitro cytotoxicity assay showed that the cell viability was significantly reduced in a dose-dependent manner following treatment with crude ethyle alcohole extract and Cholophorm and n-Butanol fractions. Based on the probit regression model, antiproliferative activities of crude ethyle alcohole extract, Cholophorm fraction, and n-Butanol fraction on Hela and AGS cells and HDFs cells were significantly different (P < 0.001). The results of flow cytometric analysis showed that crude ethyle alcohole extract and two fractions of Q. brantii acorn induced early apoptotic cell death. These findings suggest that crude ethyle alcohole extract and Cholophorm and n-Butanol fractions of Q. brantii acorn suppress the proliferation of cancer cells through induction of early apoptosis.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Ensaios de Seleção de Medicamentos Antitumorais / Extratos Vegetais / Células HeLa / Química / Apoptose / 1-Butanol / Quercus / Linhagem Celular Tumoral / Proliferação de Células Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Inglês Revista: Chinese Journal of Natural Medicines (English Ed.) Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Ensaios de Seleção de Medicamentos Antitumorais / Extratos Vegetais / Células HeLa / Química / Apoptose / 1-Butanol / Quercus / Linhagem Celular Tumoral / Proliferação de Células Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Inglês Revista: Chinese Journal of Natural Medicines (English Ed.) Ano de publicação: 2016 Tipo de documento: Artigo