Quercetin promotes neurite growth through enhancing intracellular cAMP level and GAP-43 expression / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 667-672, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-812497
ABSTRACT
The present study was designed to investigate the role of quercetin on neurite growth in N1E-115 cells and the underlying mechanisms. Quercetin was evaluated for its effects on cell numbers of neurites, neurite length, intracellular cAMP content, and Gap-43 expression in N1E-115 cells in vitro by use of microscopy, LANCE(tm) cAMP 384 kit, and Western blot analysis, respectively. Our results showed that quercetin could increase the neurite length in a concentration-dependent manner, but had no effect on the numbers of cells. Quercetin significantly increased the expression of cellular cAMP in a time- and concentration-dependent manner. The Gap-43 expression was up-regulated in a time-dependent manner. In conclusion, quercetin could promote neurite growth through increasing the intracellular cAMP level and Gap-43 expression.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Quercetina
/
Extratos Vegetais
/
Transdução de Sinais
/
Linhagem Celular
/
Neuritos
/
AMP Cíclico
/
Proteína GAP-43
/
Metabolismo
/
Regeneração Nervosa
Idioma:
Inglês
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2015
Tipo de documento:
Artigo
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