Screening of natural compounds with neuronal differentiation promoting effects in a cell-based model / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 602-608, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-812505
ABSTRACT
The purpose of this study was to establish a drug screening method for small molecules extracted from traditional Chinese medicines (TCM) that have neuronal differentiation promoting effects, using P19 embryonic carcinoma cell as a cell-based model. First, the constructed plasmid (pTα1-Luc) was transfected into P19 cells to establish a screening model. Second, several TCMs were screened using the established model and all-trans-retinoic acid as a positive control. Finally, the underlying molecular mechanism was explored using immunofluorescence staining, qT-PCR, and Western blot analysis. Our results indicated that the drug screen model was established successfully and that both honokiol and hyperoside induced P19 differentiation into neurons, with the possible molecular mechanism being modulating the Wnt signaling pathway. In conclusion, the drug screening model developed in the present study provides a rapid, cell-based screening platform for identifying natural compounds with neuronal differentiation effects.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Quercetina
/
Tretinoína
/
Compostos de Bifenilo
/
Medicamentos de Ervas Chinesas
/
Diferenciação Celular
/
Lignanas
/
Linhagem Celular Tumoral
/
Proliferação de Células
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2015
Tipo de documento:
Artigo
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