Rho kinase inhibition activity of pinocembrin in rat aortic rings contracted by angiotensin II / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 258-263, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-812696
ABSTRACT
AIM@#To investigate the effects of pinocembrin on angiotensin II (Ang II)-induced vascular contraction, and to explore its molecular mechanism of actions.@*METHODS@#The isometric vascular tone was measured in rat thoracic aortic rings with denuded endothelium. Phosphorylation level of myosin phosphatase target unit 1 (MYPT1), and protein levels of Rho kinase 1 (ROCK1, ROKβ or p160ROCK) and angiotensin II type-1 receptor (AT1R) were determined by Western blot analysis.@*RESULTS@#Pinocembrin produced a relaxant effect on endothelium-denuded aortic rings contracted by Ang II (100 nmol·L(-1)) in a dose-dependent manner. In endothelium-denuded aortic rings stimulated by Ang II, pretreatment with pinocembrin (25 and 100 μmol·L(-1)) for 20 min significantly attenuated MYPT1 phosphorylation and ROCK1 protein levels. Meanwhile, the protein level of AT1R in response to Ang II was not affected by pinocembrin in rat aortic rings.@*CONCLUSION@#These findings indicate that pinocembrin inhibits vasoconstriction induced by Ang II in rat endothelium-denuded aortic rings, and the mechanism at least in part, is due to the blockade of the RhoA/ROCK pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aorta
/
Farmacologia
/
Vasoconstrição
/
Técnicas In Vitro
/
Angiotensina II
/
Transdução de Sinais
/
Ratos Sprague-Dawley
/
Flavanonas
/
Quinases Associadas a rho
/
Genética
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS