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Effects of obesity on global genome DNA methylation and gene imprinting in mouse spermatozoa / 中华男科学杂志
National Journal of Andrology ; (12): 488-496, 2017.
Artigo em Chinês | WPRIM | ID: wpr-812736
ABSTRACT
Objective@#To investigate the influence of high fat diet-induced obesity (HFDIO) on the differentially methylated region (DMR) of the imprinted gene and global genome methylation of sperm DNA.@*METHODS@#We performed bisulfite sequencing on the DMR of the imprinted gene and global genome methylation of sperm DNA in the mouse model of HFDIO.@*RESULTS@#No statistically significant differences were found between the HFDIO model and normal control mice in MEG3-IG (93.73 vs 97.26%, P = 0.252), H19 (98.00 vs 97.83%, P = 0.920), IGF2 (97.34 vs 96.25%, P =0.166), IGF2R (1.43 vs 1.11%, P = 0.695), PEG3 (0.19 vs 0.38%, P = 0.537), MEST (0.23 vs 0.68%, P = 0.315), NNAT (0.31 vs 0.00%, P = 0.134), or SNRPN (1.88 vs 3.13%, P = 0.628). A total of 8 942 DMRs were detected across the sperm genome (P <0.05). Gene functional enrichment analysis indicated that the enriched terms with the largest numbers of genes were the metabolic process (n = 1 482), RNA synthesis (n = 779), and transcription (n = 767).@*CONCLUSIONS@#The methylation level underwent no significant change in the DMRs of the imprinted genes from the mice with HFDIO, but the CG methylation of the genes involved in the metabolic process, RNA synthesis and transcription were significantly altered.
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Espermatozoides / RNA / Fator de Crescimento Insulin-Like II / Genoma / Impressão Genômica / Metilação de DNA / Dieta Hiperlipídica / Genética / Metabolismo / Obesidade Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: National Journal of Andrology Ano de publicação: 2017 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Espermatozoides / RNA / Fator de Crescimento Insulin-Like II / Genoma / Impressão Genômica / Metilação de DNA / Dieta Hiperlipídica / Genética / Metabolismo / Obesidade Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Chinês Revista: National Journal of Andrology Ano de publicação: 2017 Tipo de documento: Artigo