Impact of deep slow-wave sleep deprivation on oxidative stress response of the testis tissue in rats / 中华男科学杂志
National Journal of Andrology
;
(12): 687-691, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-812895
ABSTRACT
Objective@#To investigate the influence of deep slow-wave sleep deprivation on the oxidative stress of testicular tissue in rats.@*METHODS@#Thirty-six 5-week-old male Wistar rats were equally randomized into deep slow-wave sleep deprivation group (SD1), deep slow-wave sleep and duration sleep deprivation group ( SD2), and a cage control group (CC). The rat model of deep slow-wave sleep deprivation was established using the flowerpot technique. The rats in the SD1 group were interfered every 24 minutes and deprived of 12 hours of sleep at night, those in the SD2 group deprived of 8 minutes of sleep at an interval of 24 minutes and 12 hours of sleep at night, and those in the CC group exposed to 12 hours of daylight and 12 hours of darkness. After 28 days, all the rats were executed for measurement of the testis volume and protein content, determination of the methane dicarboxylic aldehyde (MDA) level and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and observation of the pathological changes in the testicular tissue under the microscope.@*RESULTS@#Compared with the CC group, the rats in the SD1 and SD2 groups showed significantly reduced body weight ([268.5 ± 1.6] vs [248.1 ± 25.1]and[232.9 ± 10.1]g, P0.05). The lumens in the testis were narrowed, with obvious hyperplasia, hyperemia and edema in the peripheral interstitial tissue, but no significant pathologic changes were observed in the testis tissue of the SD1 group.@*CONCLUSIONS@#Long-term deprivation of deep slow-wave sleep impairs the structure of the testis tissue and induces oxidative stress response in rats.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Privação do Sono
/
Fases do Sono
/
Superóxido Dismutase
/
Testículo
/
Fatores de Tempo
/
Peso Corporal
/
Redução de Peso
/
Distribuição Aleatória
/
Ratos Wistar
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2017
Tipo de documento:
Artigo
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