THAP11 mediates the proliferation and apoptosis of esophageal cancer cells via inhibiting ubiquitination of p53 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1338-1343, 2019.
Artigo
em Chinês
| WPRIM
| ID: wpr-813009
ABSTRACT
To investigate the effects of thanatos-associated protein 11 (THAP11) on the proliferation and apoptosis of esophageal cancer cell and the underlying mechanism.
Methods:
Expression of THAP11 in human esophageal epithelial cells (Het-1A) and esophageal cancer cells (Eca109, TE-1, Ec 9706) were detected by Western blotting. Esophageal cancer TE-1 cells were divided into 3 groups a normal control (NC) group, a negative control (LV-NC) group and a THAP11 (LV-THAP11) group. Then the cell proliferation were detected by MTT assay, cell apoptosis were detected by flow cytometry, caspase-3 and caspase-9 levels were detected by caspases kits. Ubiquitination of p53 was determined in esophageal cancer TE-1 cells.Results:
Expression of THAP11 was reduced in esophageal cancer cells compared with human esophageal epithelial cells (P<0.05). After transfection with LV-THAP11 in TE-1 cells, cell viability was reduced (P<0.05), while apoptosis rate and caspase-3 and caspase-9 levels were increased (P<0.05), indicating that THAP11 inhibited growth of esophageal cancer cells. In addition, the THAP11 increased the levels of p53 (P<0.05) and inhibited the ubiquitination of p53 regulated by MDM2.Conclusion:
THAP11 may inhibit the proliferation of esophageal cancer cells by inhibiting ubiquitination of p53.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Repressoras
/
Neoplasias Esofágicas
/
Proteína Supressora de Tumor p53
/
Apoptose
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Ubiquitinação
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2019
Tipo de documento:
Artigo
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