Effects of MnSOD silence on in vitro tumorigenicity in NCI-H446 cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 583-588, 2018.
Artigo
em Chinês
| WPRIM
| ID: wpr-813223
ABSTRACT
To investigate the effect of manganese superoxide dismutase (MnSOD) silence on the in vitro tumorigenicity in human small cell lung cancer NCI-H446 cells and the underlying mechanisms.
Methods:
Sphere formation cells from NCI-H446 cells were obtained by suspension culture, while the expression of MnSOD and urokinase type plasminogen activator (uPAR) was analyzed by Western blot. Silence of MnSOD was performed by adenovirus infection in the second passage formation cells, and the effect of MnSOD silence on tumorigenicity in NCI-H446 cells was evaluated by sphere formation assay and soft-agar colony formation assay, while the expression of uPAR was analyzed by Western blot.Results:
Compared with NCI-H446 cells, the sphere formation rate, colony formation rate, and the expression of MnSOD and uPAR were significantly increased in the second passage sphere formation cells in NCI-H446 cells (P<0.05). Silence of MnSOD inhibited the sphere formation rate, colony formation rate, and the expression level of uPAR in the second passage sphere formation cells in NCI-H446 cells.Conclusion:
MnSOD may promote tumorigenicity in NCI-H446 cells by up-regulation of uPAR expression in vitro.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Superóxido Dismutase
/
Técnicas In Vitro
/
Ensaio Tumoral de Célula-Tronco
/
Regulação para Cima
/
Adenoviridae
/
Esferoides Celulares
/
Interferência de RNA
/
Linhagem Celular Tumoral
/
Carcinoma de Pequenas Células do Pulmão
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2018
Tipo de documento:
Artigo
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