Expression of exogenous gene in bone marrow stromal cells by liposome / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 140-144, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-813418
ABSTRACT
OBJECTIVE@#To transform eukaryotic expression vector pEGFP-PDX-1 into marrow stromal cells by liposome and to optimize the conditions of transformation.@*METHODS@#The recombinant vector was identified by enzyme digestion analysis and sequencing. The recombinant plasmid was transformed into bone marrow stromal cells and it changed the quantity of DNA or liposome. The expression of PDX-1 gene in the transformed cells was detected by immunocytochemical staining.@*RESULTS@#Enzyme digestion analysis and sequencing showed that the interesting gene was integreted into the recombinant vector. We obtained satisfactory efficiency of transfection when the ratio of DNA and liposome was 1 1 or 1 2. The PDX-1 in the transformed cells was expressed by immunocytochemical staining.@*CONCLUSION@#The eukaryotic expression vector pEGFP-PDX-1 was constructed for the first time in China. We have enhanced the efficiency of transfection by optimizing the transformation conditions. It is possible to use the bone marrow stromal cells as seed cells in tissue-engineering.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Células da Medula Óssea
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Transfecção
/
Transativadores
/
Células Cultivadas
/
Células Estromais
/
Ratos Sprague-Dawley
/
Proteínas de Homeodomínio
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2005
Tipo de documento:
Artigo
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