Construction of two hepatocellular carcinoma cell models for the expression of HBV X gene with different selection characteristics / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 636-639, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-813459
ABSTRACT
OBJECTIVE@#To construct 2 hepatocellular carcinoma (HCC) cell models for the expression of HBV X gene with different selection characteristics.@*METHODS@#HepG2 HCC cells were infected with eukaryotic expression vectors with HBV X gene, pCEP4-X, and pcDNA3. 1 (+)-X. Single cell clone was selected by hygromycin and neomycin. After propagating culture for certain periods, the HBV X gene expression was identified by PCR, RT-PCR, and Western blot.@*RESULTS@#Single HCC cell clone with HBV X gene transferred resistant to hygromycin and neomycin was selectively cultured, and the cells could be propagated for certain periods. PCR, RT-PCR, and Western blot identified the expression of HBV X gene.@*CONCLUSION@#Two HCC cell models for the expression of HBV X gene with different selection characteristics have been successfully constructed.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Transativadores
/
Carcinoma Hepatocelular
/
Linhagem Celular Tumoral
/
Células Eucarióticas
/
Vetores Genéticos
/
Genética
/
Neoplasias Hepáticas
/
Metabolismo
/
Modelos Biológicos
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2005
Tipo de documento:
Artigo
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