Effect of androgen on nitric oxiode synthase activity in the kidney of rats with ischemic injury / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To investigate the effect of androgen on nitric oxide synthase activity and kidney function in the kidney of rats with ischemic injury.@*METHODS@#Fourty-eight adult healthy male SD rats were randomly divided into the renal ischemia group (n = 24 ) and the orchidectomized group with renal ischemia (n = 24). The two groups were randomly divided into 4 groups by the survival time 0, 1, 3 and 7 days. NADPH diaphorase histochemistry was used to measure the nitric oxide synthase activity of the kidney with ischemic injury in the orchidectomized group and the control group. The image analysis technology system was used to quantify the NOS. BUN and BCr in the plasma were detected when the rats were executed.@*RESULTS@#The NOS histochemical staining of the kidney was mainly distributed in the renal tubule, macula densa and low in the renal glomerular. The NOS activity of macula densa significantly decreased in the orchidectomized group compared with that in the control group (P 0.05) , but on the third day the data markedly increased (P < 0.05).@*CONCLUSION@#Androgen can adjust the blood flux of glomerular by regulating the NOS activity of macula densa, and affect the kidney function.