Reciprocal Activation of Fibroblast-like Synoviocyte and Type II Collagen-reactive T cell in Rheumatoid Arthritis / 대한류마티스학회지
The Journal of the Korean Rheumatism Association
;
: 25-36, 2004.
Artigo
em Coreano
| WPRIM
| ID: wpr-81390
ABSTRACT
OBJECTIVE:
To investigate the interaction between type II collagen (CII)-reactive T cell and fibroblast-like synoviocyte in rheumatoid arthritis (RA).METHODS:
Peripheral blood T cells from RA patients were cultured with bovine CII and analyzed by flow cytometry. After co-culture with CII-reactive T cells and fibroblast-like synoviocytes (FLS), the expression of cytokines (IL-15 and TNF-alpha from FLS, IFN-gamma and IL-17 from CII-reactive T cells) were determined by ELISA and RT-PCR.RESULTS:
CII-reactive T cells expressed CD69, one of the early activation markers, and produced significant amount of IFN-gamma, and proliferated. IL-15 and TNF-alpha expression from FLS were significantly elevated when co-culture with CII-reactive T cells and inhibited by physical interruption of cell-to-cell contact or anti-CD40 antibody. IFN-gamma and IL-17 expression from CII-reactive T cells were also significantly elevated when co-culture with FLS and inhibited by anti-IL-15 monoclonal antibody.CONCLUSIONS:
CII-reactive T cells can activate FLS to secret proinflammatoy cytokines and interactions between these two cells drive further activation of each other. These data suggest that CII-reactive T cell may play a important role in pathogenesis of RA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Artrite Reumatoide
/
Ensaio de Imunoadsorção Enzimática
/
Linfócitos T
/
Citocinas
/
Fator de Necrose Tumoral alfa
/
Técnicas de Cocultura
/
Interleucina-15
/
Interleucina-17
/
Colágeno Tipo II
/
Citometria de Fluxo
Limite:
Humanos
Idioma:
Coreano
Revista:
The Journal of the Korean Rheumatism Association
Ano de publicação:
2004
Tipo de documento:
Artigo
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