Differential proteomic analysis of nasopharyngeal carcinoma cell lines 5-8F and 6-10B / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 978-984, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-813964
ABSTRACT
OBJECTIVE@#To compare the proteome difference of nasopharyngeal carcinoma (NPC) cell lines 5-8F and 6-10B, and to screen these proteins associated with NPC metastasis.@*METHODS@#Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins from NPC cell lines 5-8F and 6-10B with different metastatic potentials and same genetic background, respectively. PDQuest software was applied to analyze 2-DE images, and the differentially expressed protein spots between 5-8F and 6-10B were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The expression levels of partial identified proteins in the 2 cell lines were detected by Western blot.@*RESULTS@#2-DE maps of total proteins from 5-8F and 6-10B were established. A total of 65 differential protein spots in the 2 cell lines were found, and 15 non-redundant differential expression proteins were identified by MALDI-TOF-MS. Western blot showed that Annexin A1 and 14-3-3 protein sigma were differential expression proteins in 5-8F and 6-10B, which was consistent with the Results from the comparative proteomic analysis.@*CONCLUSION@#Fifteen non-redundant differential expression proteins are useful for studying the metastatic mechanism of NPC.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Espectrometria de Massas
/
Carcinoma
/
Eletroforese em Gel Bidimensional
/
Neoplasias Nasofaríngeas
/
Proteoma
/
Proteômica
/
Linhagem Celular Tumoral
/
Carcinoma Nasofaríngeo
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2007
Tipo de documento:
Artigo
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