Effect of mycophenolate mofetil on the expression of early inflammatory reaction in diabetic rats / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To investigate the effect of mycophenolate mofetil(MMF) on early inflammatory reaction of renal lesion in streptozotocin(STZ)-induced diabetic rats.@*METHODS@#Thirty-six male Sprague-Dawley rats were randomly divided into 3 groups after uninephrectomy normal control group, diabetic model group, and MMF-treated group. Six rats in each group were sacrificed at the 4th week and 14th week after STZ injection. Twenty-four hour urinary protein (24 h Upro) count was measured before death. The expressions of regulated on activation of normal T expressed and secreted (RANTES),ectodermal dysplasia (ED-1)and Col-IV protein in the renal tissue were detected by immunohistochemistry. The expression of RANTES mRNA in the renal tissue was detected by RT-PCR.@*RESULTS@#MMF prevented the increasing of 24h Upro in diabetic rats,and the expressions of RANTES,ED-1,Col-IV protein and RANTES mRNA in the kidney of MMF-treated rats were significantly decreased.@*CONCLUSION@#MMF plays an early renal protective role in diabetic nephropathy, possibly through inhibition of early inflammatory reaction.