Effect of sodium tungstate on glucose metabolism in adipocytes / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 727-730, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-814007
ABSTRACT
OBJECTIVE@#To explore the effect of sodium tungstate on glucose metabolism in adipocytes and its mechanism.@*METHODS@#After 3T3-L1 preadipocytes were differentiated into adipocytes, these adipocytes were incubated with sodium tungstate (0, 150, 300, 500, and 700 micromol/L) for 48 h, and then glucose consumption of the adipocytes was detected by glucose-oxidase assay. Glucose transport was determined by the uptake of 2-deoxy-[3H]-D-glucose, and the expression of glucose transport-4 (GLUT-4) mRNA was identified by semi-quantitative RT-PCR.@*RESULTS@#Sodium tungstate (150 approximately 700 micromol/L) could significantly increase the glucose consumption and glucose transport with a concentration dependent-effect. Sodium tungstate could increase GLUT-4 mRNA expression.@*CONCLUSION@#Sodium tungstate can enhance the glucose metabolism of adipocytes by up-regulating the expression of GLUT-4 mRNA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
RNA Mensageiro
/
Regulação para Cima
/
Compostos de Tungstênio
/
Adipócitos
/
Células 3T3-L1
/
Transportador de Glucose Tipo 4
/
Genética
/
Glucose
/
Hipoglicemiantes
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2008
Tipo de documento:
Artigo
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