Effeet of rapamycin on mTOR and eIF-4E expression in coxsackievirus B3-induced rat myocardial cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 612-617, 2008.
Article
em Zh
| WPRIM
| ID: wpr-814028
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To observe the effeet of rapamycin, an inhibitor of mammalian target of rapamycin (mTOR), on mTOR and eukaryotic initiation factor-4E(eIF-4E)expression in coxsac-kievirus B3 (CVB3)-induced rat myocardial cells and to investigate the role of mTOR/eIF-4E signal pathway in viral myocarditis.@*METHODS@#To construct a cell model of viral myocarditis with primary cultured myocardial cells. Myocardial cells infected by CVB3 were treated with 10 nmol/L rapamycin according to the cell toxicity test. The mTOR and eIF-4E expressions of cells were determined by RT-PCR and Western Blot.@*RESULTS@#Rapamycin inhibited the degeneration of CVB3-induced myocardial cells. Expressions of mTOR and eIF-4E mRNA or protein in CVB3-induced myocardial cells were significantly upregulated compared with the control group (P < 0.05), and rapamycin (10 nmol/L) inhibited the upregulation (P < 0.05).@*CONCLUSION@#Rapamycin can downregulate the expressions of mTOR and eIF-4E in CVB3-induced myocardial cells, suggesting that mTOR/eIF-4E signal transduction may play an important role in viral myocarditis.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Proteínas Quinases
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Virologia
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Transdução de Sinais
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Ratos Sprague-Dawley
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Enterovirus Humano B
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Sirolimo
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Infecções por Coxsackievirus
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Miócitos Cardíacos
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Fator de Iniciação 4E em Eucariotos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2008
Tipo de documento:
Article