Preparation and preliminary application of colloidal carbon dipstick for schistosomiasis japonica / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1063-1069, 2009.
Artigo
em Inglês
| WPRIM
| ID: wpr-814192
ABSTRACT
OBJECTIVE@#To develop a rapid and simple immunoassay to detect antibodies in the sera of patients infect Schistosoma japonicum (S. japonicum).@*METHODS@#Soluble egg antigen (SEA) of S. japonicum conjugated with colloidal carbon in advance was used to react with the antibodies in the sera of patients with schistosomiasis. Then the carbon-antigen-antibody complex would be captured by SEA which had been absorbed on the nitrocellulose membrane and a gray band was shown.@*RESULTS@#A total of 137 sera samples from S. japonicum epidemic area were tested, and the consistency, sensitivity, and specificity of colloidal carbon dipstick assay were 98.54%, 98.99%, and 97.37%, respectively, compared with the IHA method. The gray scale of bands on the dipstick was curvilinear to serum titer which revealed that the assay could be used semi-quantitatively in serum analysis.@*CONCLUSION@#Colloidal carbon dipstick assay is not only rapid and simple, but also sensitive and specific for the detection of serum antibodies of schistosomiasis japonica. It will be a practical immunological assay for the diagnosis of schistosomiasis in the field testing.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Schistosoma japonicum
/
Sangue
/
Esquistossomose Japônica
/
Imunoensaio
/
Carbono
/
Anticorpos Anti-Helmínticos
/
Química
/
Sensibilidade e Especificidade
/
Coloides
/
Diagnóstico
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2009
Tipo de documento:
Artigo
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