Effect of gamma-aminobutyric acid on malignant phenotypes of hepatocellular carcinoma cell line HepG-2 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 752-756, 2009.
Article
em Zh
| WPRIM
| ID: wpr-814278
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To determine the effect of gamma-aminobutyric acid(GABA) on proliferation and malignant phenotypes of hepatocellular carcinoma cell line HepG-2.@*METHODS@#HepG-2 cells were cultured by routine method, and then treated with different concentrations of GABA. The proliferation of HepG-2 cells was measured through MTT, doubling time and cell cycles by flow cytometry. The malignant phenotypes were investigated by soft agar colony formation assay.@*RESULTS@#Compared with the control group, GABA efficiently stimulated the proliferation of HepG-2 cells in a dose-dependent manner and affected the distribution of cell cycles of HepG-2 cells. The doubling time of the control group and the GABA-treated group were 39.0, 30.6, 30.0, 27.3, 26.6, and 38.2 h, respectively. The colony formation rates were 3.2%, 4.2%, 5.4%, 6.6%,6.5%, and 3.5%, respectively. Tumorigenicity testing showed that the average weights of tumor was 1.382 g, and 0.285 g for the 2 groups. The difference between the control group and the GABA-treated groups was significant (P<0.01).@*CONCLUSION@#GABA can enhance the proliferation and malignant phenotypes of HepG-2 cells.
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Assunto principal:
Farmacologia
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Fenótipo
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Ciclo Celular
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Proliferação de Células
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Relação Dose-Resposta a Droga
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Células Hep G2
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Ácido gama-Aminobutírico
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Camundongos Endogâmicos BALB C
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Camundongos Nus
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Transplante de Neoplasias
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2009
Tipo de documento:
Article