Mechanism of Smad 3 signaling pathway and connective tissue growth factor in the inhibition of form deprivation myopia by pirenzepine / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 349-355, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-814322
ABSTRACT
OBJECTIVE@#To observe the inhibitive effect of pirenzepine on form deprivation myopia in guinea pigs and to explore the mechanism of Smad3 signaling pathway and connective tissue growth factor (CTGF) in the inhibition of myopia by pirenzepine.@*METHODS@#Forty 1-week-old guinea pigs of either sex were randomly divided into 4 groups a control group (Group I), a form deprivation group (Group II), a pirenzepine ophthalmic solution group (Group III), and a sodium chloride ophthalmic solution group (Group IV). Translucent blinders were used in the right eyes of Group II, III and IV. The left eyes were not given any treatment as the normal control group. Covered eyes of Group III and IV were given 3% pirenzepine ophthalmic solution and 0.1% azone ophthalmic solution respectively twice every day. Six weeks later, refraction and axial length were measured at the end of the experiment, and immunohistochemistry and Western blot were used to analyze the expression levels of Smad3 and CTGF in the sclera of all 4 groups.@*RESULTS@#There was no significant difference between Group III and I in relative refraction and changes of axial length (P>0.05). The difference of Group II and IV compared with Group I was statistically significant (P0.05), while the difference in Group II, IV and I was significant (P0.05).@*CONCLUSION@#Pirenzepine ophthalmic solution can inhibit the development of form deprivation myopia. Pirenzepine may affect Smad3 signaling pathway in the sclera by inhibiting the development of form deprivation myopia.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Privação Sensorial
/
Pirenzepina
/
Transdução de Sinais
/
Distribuição Aleatória
/
Antagonistas Muscarínicos
/
Proteína Smad3
/
Fator de Crescimento do Tecido Conjuntivo
/
Cobaias
/
Metabolismo
/
Miopia
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2009
Tipo de documento:
Artigo
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