Change of p-CREB after surgical incisional pain in rat spinal cord / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 833-839, 2010.
Article
em Zh
| WPRIM
| ID: wpr-814387
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To explore the role of phosphorylation of cAMP response element binding protein (CREB) in the incision-induced pain hypersensitivity.@*METHODS@#A longitudinal incision was made in one plantar hind paw of isoflurane-anesthetized rats. Spinal cords were removed at various postoperative time after behavior test. Phosphorylation of CREB was determined by immunohistochemistry and double-labeling immunofluorescence. Morphine and gabapentin were intraperitoneally injected before the behavior test and were used to determine the interaction between phosphorylation of CREB and morphine and gabapentin.@*RESULTS@#After the hind-paw incision, phosphorylation of CREB was enhanced in the ipsilateral lumbar spinal cord (P0.05). Gabapentin didn't inhibit the phosphorylation of CREB (P<0.05) but partly inhibited the mechanical allodynia.@*CONCLUSION@#Incision induces the phosphorylation of CREB in the spinal cord, and the increase of p-CREB is mainly in the neurons. Phosphorylation of CREB in the spinal cord contributes to the pain hypersensitivity induced by surgical incision.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Dor Pós-Operatória
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Fosforilação
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Medula Espinal
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Cirurgia Geral
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Ratos Sprague-Dawley
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Limiar da Dor
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Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico
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Pé
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Metabolismo
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Neurônios
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2010
Tipo de documento:
Article