Involvement of JNK signal transduction pathway in endothelial cell apoptosis induced by intermittent high glucose / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 616-621, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-814409
ABSTRACT
OBJECTIVE@#To evaluate the effect of intermittent high glucose on the apoptosis of human umbilical vein endothelial cells (HUVECs) and its mechanism.@*METHODS@#Intermittent high glucose and constant high glucose were applied to HUVEC-12 for 7 days. Flow cytometer and fluorescent staining with Hoechst 33258 were used to detect apoptosis of HUVEC-12. The superoxide dismutase (SOD) activity and the content of malonaldehyde (MDA) in culture solution were detected with colorimetry, and the changes of p-JNK level were examined by Western blot.@*RESULTS@#The apoptosis rate was obviously higher in the intermittent high glucose group than that in the constant high glucose group (P < 0.05). The SOD activity was significantly lower in the intermittent high glucose group (P < 0.05), but MDA level was higher than those of constant high glucose(P < 0.05). SP600125, the inhibitor of JNK, decreased the apoptosis rate induced by intermittent high glucose (P < 0.05). Antioxidant (Vitamin C) inhibited the p-JNK, decreased the apoptosis rate (P < 0.05).@*CONCLUSION@#Intermittent high glucose is easier to worsen the proapoptotic effects on HUVECs than that of constant high glucose, which may account for the increased oxidative stress, and then activates JNK signal transduction pathway.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Fisiologia
/
Veias Umbilicais
/
Transdução de Sinais
/
Células Cultivadas
/
Apoptose
/
Biologia Celular
/
Células Endoteliais
/
Proteínas Quinases JNK Ativadas por Mitógeno
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2010
Tipo de documento:
Artigo
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