Effect of intermittent high glucose on synthesis of nitric oxide in human umbilical vein endothelial cells and its mechanism / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 295-300, 2010.
Artigo
em Inglês
| WPRIM
| ID: wpr-814451
ABSTRACT
OBJECTIVE@#To compare the effect of intermittent and constant high glucose media on the synthesis of vascular relaxing factor nitric oxide (NO) in cultured human umbilical vein endothelial cells (HUVECs), and to investigate its mechanism.@*METHODS@#HUVECs were exposed to 5.5 mmol/L glucose (normal control), 20 mmol/L glucose (constant high glucose), and 5.5 mmol/L alternating with 20 mmol/L glucose (intermittent high glucose) for 7 days. The NO level was measured using Griess Reaction in cell culture supernatants; the expressions of PI3K, PKB and eNOS mRNA and protein were measured with RT-PCR or Western blot.@*RESULTS@#NO level in the intermittent high glucose group was significantly lower than that in the constant high glucose group (P<0.05). The expression of PI3K, PKB, and eNOS in the intermittent high glucose group was significantly lower than that in the constant high glucose group (P<0.05).@*CONCLUSION@#Intermittent high glucose could be more deleterious to endothelial cells than constant high glucose, and may lead to decrease synthesis of NO by inhibiting PI3K/PKB/eNOS pathway activation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Veias Umbilicais
/
RNA Mensageiro
/
Células Cultivadas
/
Fosfatidilinositol 3-Quinases
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Meios de Cultura
/
Biologia Celular
/
Células Endoteliais
/
Óxido Nítrico Sintase Tipo III
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2010
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS