Proteomic analysis of human ovarian cancer paclitaxel-resistant cell lines / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 286-294, 2010.
Article
em En
| WPRIM
| ID: wpr-814452
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To study the proteins related to paclitaxel-resistant of ovarian cancer cell line.@*METHODS@#The total proteins of paclitaxel-resistant and paclitaxel-sensitive human ovarian cancer cell lines were separated by 2-dimensional gel electrophoresis (2-DE). The differentially expressed proteins were analyzed using image analysis software. The differential proteins were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins.@*RESULTS@#Forty differentially expressed proteins were found by image analysis software, and 24 differential proteins were identified by mass spectrometry. These proteins included proliferation cell nuclear antigen (PCNA), nm23, prohibitin (PHB), annexin, alpha-enolase, heat shock protein (HSP), and so on.@*CONCLUSION@#Twenty-four proteins in human ovarian cancer cell lines of paclitaxel-resistant and paclitaxel-sensitive are found by proteomic techniques, which may be involved in the paclitaxel-resistance of human ovarian cancer cells.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Neoplasias Ovarianas
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Patologia
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Farmacologia
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Proteínas Repressoras
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Eletroforese em Gel Bidimensional
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Paclitaxel
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Antígeno Nuclear de Célula em Proliferação
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Resistencia a Medicamentos Antineoplásicos
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Proteoma
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Proteômica
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2010
Tipo de documento:
Article