Purification and identification of HMGB1 secreted by liver cells and immune cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1065-1070, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-814475
ABSTRACT
OBJECTIVE@#To purify and identify HMGB1 secreted by liver cells HepG2 and immune cells U937.@*METHODS@#We cultured the liver cell lines HepG2 and immune cell lines U937, and stimulated them with HMGB1 (400 ng/mL) for 20 h. Then the supernatant was collected. Ultrafiltration centrifugation, CM-Sepharose cation, DEAE-Sepharose anion exchange chromatography, Sephadex G75-gel filtration chromatography, and immunoprecipitation were used for purification. The molecular weight and identity of HMGB1 was confirmed by SDS-PAGE and Western blot.@*RESULTS@#A sharp stained protein band with a molecular weight of about 26 kD was obtained by SDS-PAGE analysis and shown to be HMGB1 confirmed by Western blot.@*CONCLUSION@#High purified HMGB1 can be separated from these two cell lines.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Monócitos
/
Técnicas de Cultura de Células
/
Células U937
/
Hepatócitos
/
Proteína HMGB1
/
Eletroforese em Gel de Poliacrilamida
/
Células Hep G2
/
Metabolismo
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2011
Tipo de documento:
Artigo
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