Recombinant expression of hepatoma associated gene and its protein function / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 655-661, 2011.
Article
em Zh
| WPRIM
| ID: wpr-814536
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To recombinant express hepatoma associated gene(HTA) and pre-test the function of HTA to determine the role of HTA in the development of liver cancer.@*METHODS@#HTA338-616 was amplified from HepG2 cells and cloned into the prokaryotic expression vector pET21a(+)-MBP. The proteins MBP and MBP-HTA were induced, purified by His-tag magnetic bead purification kit and identified by Western blot and ELISA. HepG2 cells were stimulated with MBP or MBP-HTA proteins. MTT assay and colony formation assay were employed to examine the proliferation of these cells and the changes of cell cycle distribution were determined by flow cytometry.@*RESULTS@#The prokaryotic expression plasmid pET21a(+)-MBP-HTA was successfully constructed. We got a 52 kD purified purpose protein.The proliferation of HepG2 cells stimulated with MBP-HTA was significantly higher than those stimulated with MBP and negative controls. HepG2 cells stimulated with MBP-HTA showed significant decrease fraction in G1 phase and increase fraction in S phase, and the cell proliferation was enhanced.@*CONCLUSION@#HTA protein can significantly promote the proliferation of HepG2 cells, which may be related to the promotion of G1 phase to S phase.
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1
Índice:
WPRIM
Assunto principal:
Farmacologia
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Fisiologia
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Proteínas Recombinantes de Fusão
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Dados de Sequência Molecular
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Sequência de Bases
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Fases de Leitura Aberta
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Proliferação de Células
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Escherichia coli
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Genes Neoplásicos
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Células Hep G2
Tipo de estudo:
Health_technology_assessment
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2011
Tipo de documento:
Article