Regulation of invasion behavior of ERK-mediated trophoblast by focal adhesion kinase / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 559-564, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-814549
ABSTRACT
OBJECTIVE@#To investigate the role of focal adhesion kinase (FAK) in extracellular signal-regulated kinase (ERK) signaling pathway mediated invadsion of trophoblasts.@*METHODS@#We established a human extravillous cytotrophoblasts in vitro invasion model. Different concentrations of herbimycin A(FAK inhibitor)and PD98059 (ERK inhibitor) were given to observe the influence on the growth of trophoblast cells, FAK, ERK phosphorylation, and trophoblast invasion abilities.@*RESULTS@#The expression of phosphorylated FAK in the extravillous cytotrophoblasts (EVCT) was inhibited by herbimycin A in a concentration-dependent manner and expression of phosphorylated ERK1/2 was also partially reduced. PD98059 had no effect on the expression of phosphorylated FAK. Herbimycin A and PD98059 suppressed the in vitro invasion of EVCT to various degrees.@*CONCLUSION@#ERK signaling pathway may be the common pathway for many invasive signals,and play a key role in the regulation of trophoblast invasion.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fosforilação
/
Fisiologia
/
Trofoblastos
/
Flavonoides
/
Transdução de Sinais
/
Divisão Celular
/
Movimento Celular
/
Benzoquinonas
/
Rifabutina
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2011
Tipo de documento:
Artigo
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