Effects of norcantharidin on lipopolysaccharide-induced hepatocyte injury in vitro / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 285-289, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-814686
ABSTRACT
OBJECTIVE@#To study the effects of norcantharidin (NCTD) on lipopolysaccharide (LPS)-induced hepatocyte injury and the expression of TNF-α and IL-6 in vitro.@*METHODS@#Hepatocytes were isolated from male Sprague-Dawley rats by collagenase perfusion. LPS at concentration of 40 mg/L was used to induce injury to the cultured cells, and NCTD (0.5, 1.0, 2.5 μg/mL) was added at the same time. After 24 h of incubation, the cell proliferation rates were detected by MTT. LDH, TNF-α and IL-6 were measured by appropriate reagent kits.NF-κB DNA binding activity was measured.@*RESULTS@#40 mg/L LPS caused a 27% growth inhibition in primary hepatocytes. LDH leakage was 20- fold higher in NCTD-treated hepatocytes than in normal ones. TNF-α and IL-6 expression significantly increased. In cells treated with NCTD at doses of 0.5, 1.0 and 2.5 μg/mL, LDH leakage, TNF-α and IL-6 expression, and NF-κB DNA binding activity were attenuated in a dose dependent manner.@*CONCLUSION@#NCTD protects hepatocytes from injury induced by LPS; the protection is associated with suppression of the inflammatory cytokine TNF-α and IL-6.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Lipopolissacarídeos
/
NF-kappa B
/
Interleucina-6
/
Fator de Necrose Tumoral alfa
/
Ratos Sprague-Dawley
/
Compostos Bicíclicos Heterocíclicos com Pontes
/
Substâncias Protetoras
/
Hepatócitos
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS