hMLH1 gene promoter methylation in joint cartilage in patients with osteoarthritis / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 928-933, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-814762
ABSTRACT
OBJECTIVE@#To investigate the role of Human MutL homologue 1 (hMLH1) gene promoter methylation in the occurrence and development of osteoarthritis (OA).@*METHODS@#General DNA was dealt with sodium bisulfite. The methylation of hMLH1 promoter was detected by methylation-specific PCR (MSP). hMLH1 protein expression in joint cartilage was detected by immunohistochemical method.@*RESULTS@#The positive percent of hMLH1 promoter methylation in OA patients was higher than that in healthy persons (χ(2)=30.634, P<0.001); the positive percent of hMLH1 protein in OA patients was significantly lower than that in healthy persons (χ(2)=37.724, P<0.001); prmoter methylation and protein expression level of hMLH1 gene showed negative correlation (rs=-0.554, P<0.001).@*CONCLUSION@#hMLH1 promoter is hypermethlated in joint cartilage cells of OA patients. Hypermethylation may affect the protein expression of hMLH1, which might play a role in the occurrence and development of OA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoartrite
/
Proteínas Nucleares
/
Cartilagem Articular
/
Regiões Promotoras Genéticas
/
Metilação de DNA
/
Proteínas Adaptadoras de Transdução de Sinal
/
Proteína 1 Homóloga a MutL
/
Genética
/
Metabolismo
Limite:
Adolescente
/
Adulto
/
Criança
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2012
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS