Construction of special reporter to detect DNA methylation regulatory activity in FCER1G gene promoter through patch-methylation / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 120-124, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-814914
ABSTRACT
OBJECTIVE@#To construct a special luciferase reporter to detect DNA methylation regulatory activity in FCER1G gene promoter regulatory element.@*METHODS@#We constructed special full and mock methylated FCER1G gene promoter regulatory luciferase reporters by patch-methylation, and detected DNA methylation regulatory activity by comparing the luciferase activity of full-methylated luciferase reporters with mock-methylated reporters.@*RESULTS@#We successfully constructed the full and mock methylated FCER1G gene promoter regulatory luciferase reporters. The ratio of luciferase activity between the full methylated and the mock methylated was (0.36±0.07)1 (P<0.001).@*CONCLUSION@#FCER1G promoter activity is methylation-sensitive and is regulated by DNA methylation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Dados de Sequência Molecular
/
Sequência de Bases
/
Regulação da Expressão Gênica
/
Regiões Promotoras Genéticas
/
Receptores de IgE
/
Genes Reporter
/
Metilação de DNA
/
Genética
/
Luciferases
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2013
Tipo de documento:
Artigo
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