Effect of DJ-1 siRNA on biological behavior of human lung squamous carcinoma SK-MES-1 cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 7-13, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-814923
ABSTRACT
OBJECTIVE@#RNA interference technology (siRNA) was used to inhibit the expression of DJ-1 gene in lung squamous cell carcinoma SK-MES-1 cells, and the cell biological behaviors were investigated to explore the function of DJ-1 gene.@*METHODS@#A targeted DJ-1 siRNA lentiviral vector with a green fluorescent protein (GFP) as a reporter was constructed. The constructed DJ-1 siRNA and control-siRNA vectors were infected into SK-MES-1 cells as experimental (DJ-1 siRNA) and control (Control siRNA) groups, respectively. The DJ-1 protein expression was determined by Western blot. The cell proliferation capability was measured with methyl thiazolyl tetrazolium (MTT). The cell cycle was analyzed by flow cytometry. The capability of cell migration was determined by Transwell method.@*RESULTS@#Compared with control-siRNA and blank-control groups, the protein expression of DJ-1 gene was down-regulated, the capability of cell proliferation was obviously inhibited (P<0.01), the cell cycle was arrested with increased number of G1- and G2-phase cells and reduced number of S-phase cells, and the capability of cell migration was significantly decreased (P<0.01) in the DJ-1 siRNA-infected cells.@*CONCLUSION@#DJ-1 gene might play a role in promoting cell proliferation and cell migration capability in vitro in lung cancer SK-MES-1 cells.
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Carcinoma de Células Escamosas
/
Movimento Celular
/
Proteínas Oncogênicas
/
Lentivirus
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Linhagem Celular Tumoral
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2013
Tipo de documento:
Artigo
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