Y-27632 reduces the MMP2 and MMP9 expression in endothelial cell via inhibition of ROCK signal pathway / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 566-570, 2016.
Article
em Zh
| WPRIM
| ID: wpr-814997
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To explore the effect of ROCK inhibitor Y-27632 on the matrix metalloproteinase 2 and 9 (MMP2 and MMP9) gene expression and activity in tumor necrosis factor α (TNF-α)-treated human umbilical vein endothelial cell (HUVEC).
@*METHODS@#HHUVEC was divided into 3 groups, a control group, a TNF-α group, and a TNF-α plus Y-27632 group. The expressions of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), MMP2 and MMP9 were examined by real-time PCR. The MMP2/9 activity was measured by gelatin zymography.
@*RESULTS@#Compared to the control group, the mRNA expressions of ICAM-1, VCAM-1, MMP2 and MMP9 were increased TNF-α-treated cells, which were suppressed by ROCK inhibitor (P<0.01). The MMP2/9 activity was elevated in TNF-α-treated cells, which was reversed by ROCK inhibitor (P<0.05).
@*CONCLUSION@#ROCK inhibitor can suppress TNF-α-induced inflammation in endothelial cells through down-regulation of MMP2/9.
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Assunto principal:
Piridinas
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Veias Umbilicais
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Transdução de Sinais
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Regulação para Baixo
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Células Cultivadas
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Fator de Necrose Tumoral alfa
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Molécula 1 de Adesão Intercelular
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Molécula 1 de Adesão de Célula Vascular
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Metaloproteinase 2 da Matriz
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Metaloproteinase 9 da Matriz
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2016
Tipo de documento:
Article