MiR-519 inhibits gastric cancer cell activity through regulation of HuR expression / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To investigate the role of miR-519 in regulating the cell activity of gastric cancer. 
@*METHODS@#The expression of miR-519 was measured by qPCR. The protein expression of HuR was detected by Western blot. MTT assay was used to detect the cell activity of gastric cancer cells.
@*RESULTS@#The protein level of HuR in the gastric cancer cells was higher than that in the control cells. Over-expression of HuR resulted in the increase in the cell activity of gastric cancer (P<0.001). Suppression of HuR decreased the cell activity of gastric cancer (P=0.001). The miR-519 level in the gastric cancer cells was lower than that in the control cells (P=0.001). Over-expression of miR-519 decreased the protein level of HuR and the activity of gastric cancer cells.
@*CONCLUSION@#MiR-may inhibit the gastric cancer cell activity through suppression of HuR expression.