Effect of calcium channel blockers on primary cultured human urethra scar fibroblasts / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1317-1322, 2016.
Article
em Zh
| WPRIM
| ID: wpr-815091
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WPRO
ABSTRACT
To investigate effects of verapamil on primary cultured human urethral scar fibroblasts (USFs) and to provide basis for protecting the formation of urethra scar.
Methods: The cell proliferation was evaluated with the cell counting kit (CCK)-8 method after USFs were incubated various verapamil concentrations (50, 100, 150, 200, or 250 μmol/L) or solvent for 12, 24, or 48 h. The protein level of matrix metalloproteinase (MMP) was evaluated with ELISA after cells were incubated with verapamil (100 μmol/L) or solvent (control cells) for 24 h.
Results: The proliferation of USFs was obviously suppressed after verapamil treatment, which was in a dose-dependent and time-dependent manner. Meanwhile, the protein levels of MMP-2 and MMP-9 in the verapamil treatment group increased obviously compared with those of the control groups (P<0.05).
Conclusion: Calcium channel blockers may prevent the excessive formation of urethra scar by inhibiting the proliferation of urethral scar fibroblasts and enhancing the activity of MMP.
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Índice:
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Assunto principal:
Patologia
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Farmacologia
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Uretra
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Bloqueadores dos Canais de Cálcio
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Verapamil
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Regulação para Cima
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Células Cultivadas
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Cicatriz
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Metaloproteinase 2 da Matriz
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Metaloproteinase 9 da Matriz
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Ano de publicação:
2016
Tipo de documento:
Article